ALBUMIN, BOVINE
ALBUMIN, BOVINE
CAS: 68551-06-4
Molecular Formula:
ALBUMIN, BOVINE - Names and Identifiers
ALBUMIN, BOVINE - Physico-chemical Properties
| Appearance | Morphological powder |
| pKa | pK1: 10-10.3 (25°C,μ= 0.1) |
| Storage Condition | 2-8°C |
| Physical and Chemical Properties | Chemical Properties This product is the most abundant protein in plasma, accounting for 55% of the total protein. It is generally isolated from healthy human plasma or from healthy maternal placenta. pI4.7. It is soluble in water; it is stable to acid; it can be polymerized and denatured when heated, and the thermal stability is small when the concentration is large. Adding sodium chloride or fatty acid salt to the solution can improve the stability. |
| Use | The main function is to maintain the plasma colloid osmotic pressure. It can be used as a blood volume expansion agent to increase colloid osmotic pressure and increase blood volume. For the prevention or rescue of hemorrhagic shock, traumatic shock, severe burns, burns, etc. It can also be supplemented with body albumin to treat hypoproteinemia, increased brain pressure due to cirrhosis and renal disorders, and albumin deficiency in women with miscarriage or premature birth. Shock due to severe burns or other causes of blood loss. Note: patients with kidney disease intravenous drip, should not be diluted with saline. Severe anemia, heart failure patients should not be used in large amounts. |
ALBUMIN, BOVINE - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | 22 - Harmful if swallowed |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-21 |
ALBUMIN, BOVINE - Upstream Downstream Industry
| Raw Materials | Aluminum hydroxide Adsorbent DIALYSIS TUBING BENZOYLATED 32MM IGE, ANTI-HUMAN, GOAT, IGG FRAC, FITC, LQ Sodium octanoate |
ALBUMIN, BOVINE - Production method
method 1. using human placental blood as raw material
Raw material processing, impregnation extraction, salting out with human placental gamma globulin operation.
Acidic precipitation, dialysis, salting out ammonium sulfate mother liquor to remove precipitation (for gamma globulin), adjust pH 4.3-4.4, place for 1h, canvas filter to take precipitation. The precipitate is wrapped in a cellophane dialysis bag, leaving a certain volume, removing air, tying the mouth, and dialyzing the running water until the ammonium sulfate content is less than 2g/L.
Filtrate [HCl]& rarr;[pH4.3-4.4] sediment [dialysis] & rarr;[for flowing water] & rarr; Dialysate
Heat treatment, salting out, dialysate is added with sodium chloride (to make the concentration reach 8.5g/L), stirred and dissolved, canvas is filtered, the filtrate is measured for protein content, and the protein concentration is diluted with pyrogenic water to 12-20g/L. Add solid sodium caprylate to 2g/L,1mol/L hydrochloric acid to adjust pH 5-5.2, stir and dissolve, 0-5oC overnight. The next day, it is still heated in a water bath at pH 5-5.2 and 67 ℃, added with activated carbon (0.2%), stirred for 15min, heated to 69 ℃, kept warm for 1h, cooled to below 45 ℃, filtered, and took filtrate. Add sodium chloride or ammonium sulfate to 178g/L according to 1g:10 times volume, adjust pH 3.75-3.85, put it for 1h, filter gauze, press dry, and take precipitate.
Dialysate [NaCl, sodium caprylate, activated carbon] & rarr;[pH5-5.2, 67-69 ℃] filtrate [NaCl]& rarr;[pH3.75-3.85] sediment
Dialysis and adsorption precipitation are successively added with 0.5 mol/L, 1.0 mol/L and 2 mol/L sodium hydroxide, stirred into paste, adjusted to pH 7.8-8, wrapped in a cellophane dialysis bag, dialyzed in running water until the chloride ion concentration is not higher than the blank control of tap water. Combine dialysate, treat with 2-4g/L aluminum hydroxide or 20-30g/L activated carbon as adsorbent, stir for 1h, filter, and collect filtrate.
Precipitate [sodium hydroxide] & rarr;[for flowing water, pH7.8-8] dialysate [aluminum hydroxide] & rarr;[1h] filtrate
Clarify, sterilize, filter to take filtrate, adjust pH 6.6-7, dilute protein to 100g/L or 250g/L, sterilize and filter, bottle or freeze-dry after passing the inspection.
Filtrate [clarification, sterilization, filtration] & rarr;[pH6.6-7] filtrate [aseptic packaging] & rarr; Albumin finished product
Methods 2. using human plasma as raw material
Complexation and dissociation Pump human serum into a stainless steel interlayer reaction tank, turn on the stirrer, adjust the pH 8.6 with sodium carbonate solution, add 2% rivanol by equal volume, stir and stand for 2-4h, separate, supernatant for preparation of human gamma globulin, and collect complexation precipitate. Dilute the precipitate with sterilized distilled water, adjust the pH to weak acidity, add sodium chloride, stir for dissociation, and circulate and cool with tap water interlayer to obtain dissociation liquid.
Human plasma [rivanol, sodium carbonate] & rarr;[pH8.6] complex [distilled water, NaCl,HCl]& rarr;[weakly acidic, 65 ℃] dissociation solution
The separation, sterilization and dissociation liquid is separated by a basket centrifuge, and the centrifugal separation liquid is clarified and filtered with a filter press, and concentrated with an ultrafilter to obtain a concentrated solution. The concentrated solution is treated at a constant temperature of 60 ℃ for 10 hours, clarified and filtered with a pressure filter, sterilized by a cold sterilization system, and filled with albumin products after inspection.
Last Update：2024-04-10 22:29:15
ALBUMIN, BOVINE - Nature
| solubility | PBS: >40 mg/mL |
| EPA chemical information | Albumins, blood plasma (68551-06-4) |
Last Update：2024-04-09 01:59:50
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Raw Materials for ALBUMIN, BOVINE
Aluminum hydroxide
Adsorbent
DIALYSIS TUBING BENZOYLATED 32MM
IGE, ANTI-HUMAN, GOAT, IGG FRAC, FITC, LQ
Sodium octanoate
Adsorbent
DIALYSIS TUBING BENZOYLATED 32MM
IGE, ANTI-HUMAN, GOAT, IGG FRAC, FITC, LQ
Sodium octanoate